Probing Serotonin Transporter (SERT) Structure by Mutagenesis
Perez, Cheyeanne; Perez, Elizabeth;
Veeramachaneni, Rathna; Cascio, Michael
Department of Chemistry and Biochemistry,
Duquesne University
Plasmids may be used for the
introduction of recombinant DNA into a bacteria cell. Since plasmids replicate
with bacteria cells they can be amplified. Initially a transformation using the
plasmids pUC 18 and pWhitescript was performed, followed by a miniprep and
maxiprep. The transformation was used to introduce the plasmid into bacteria cells.
The mini prep was then performed to extract and purify the recombinant DNA to
confirm successful transformation. Then a maxiprep was preformed to increase
the quantity of the recombinant plasmid. The familiarity with the methods of
transformation, miniprep/maxiprep allow for further work with a plasmid
containing the sequence of the rat serotonin transporter (rSERT) to introduce
point mutations that will allow for later structural studies of the rSERT
protein in the future.
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